Answer:
Extraction of genomic DNA
Explanation:
Resuspend the cells(any number), smaller numbers also work, in 10-20 ul of TE buffer or milli Q water. boil for 5-10 min in a waterbath, quick chill, short spin. use the supernatant as a template for PCR.
If cells are very less, u can directly add cells to the PCR reaction without enzyme,boil for 5-10 min in a waterbath, let it cool slowly, short spin. Add enzyme to the supernatant and start the reaction at extension step followed by normal PCR reaction steps.
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Answer:
D. Alternative splicing is the mechanism that produce complexity in the genes by splicing some of the protein coding part (exons) of a genes
Explanation:
There are certain splicing enhancers sites present in Exons which facilitiates the binding of RNA binding protein specifically SR protein family rich in Serine and Arginine residues. This SR protein will help in splicing of exons.
The significance of this type of mechanism is the ability of a cell to produce an isoform of protein which have retain their function.
This mechanism also help is diversity or in short in evolution.
Answer:
1 is A 2 is D 3 is B 4 is C
Explanation:
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