The process of fusing two or more DNA molecules to produce a hybrid is known as recombinant DNA. Restrictions endonucleases and ligases are two classes of enzymes that enable the technique.
When a restriction endonuclease detects a particular DNA sequence, it makes cuts inside or near that sequence. The recognition sequence of a restriction enzyme will haphazardly appear on every (1–4)n bases along a random DNA chain. The equation states how many fragments ends (measured in moles) are produced when a restriction enzyme cuts DNA.
Moles of DNA ends =2x(grams of DNA)/(number of bp)(660 g/mol / bp).
The equation describes how many ends are produced when circular DNA is digested by a restriction enzyme.
Mole ends=2x(moles DNA)x number of restriction sites.
The following equation can be used to determine how many ends are produced during the digestion of a linear molecule by a restriction endonuclease.
Mole ends=2x(molesDNA)number of restriction sites +[2x(moles of DNA)].
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The protest that is present in
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cells shape, provides protection and helps keep water in its body.
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Animals and plants need to get rid of carbon dioxide gas through a process called respiration. Carbon moves from fossil fuels to the atmosphere when fuels are burned. When humans burn fossil fuels to power factories, power plants, cars and trucks, most of the carbon quickly enters the atmosphere as carbon dioxide gas.
