Question

You are performing an experiment with Primase, using a specific DNA template. In this experiment, you provide NTPs (ATP, UTP, CTP, GTP) which contained only one radioactive phosphate group, in the alpha-phosphate position (the alpha- phosphate is the one attached to the sugar. the other phosphates are beta and gamma). After RNA synthesis occurs, you separate the resultant RNA strand any of the NTPs that have not been incorporated into the RNA, and measure the radioactivity of the newly synthesized RNA.A) In this experiment, would you expect the newly synthesized RNA be radioactive? Explain why.B) If you forgot to add CTP when you did the experiment, would the resulting RNA be radioactive?C) If you did the experiment, with all the NTPs provided, but only the beta phosphate was radioactive for each of the NTPs, would radioactivity be found in the RNA? Explain why.D) If you did the experiment, with all the required NTPs, but only the ATP was radiolabelled Would radioactivity be found in the RNA? Explain why.

Answer 1

Answer:

(a). Yes.

(b). RNA becomes Radioactive.

(c). No.

(d). It depends. If the gamma phosphate and beta phosphate are not labeled, then the RNA will have certain degree of radioactivity.

Explanation:

So, let us do right into the explanation behind the answers to the questions asked.

(a). The answer to this part of the question is "Yes" because in creating or in the formation of the glycosidic bond, all the alpha phosphate group were taken into consideration that is they were all used, thus making the newly synthesized RNA to be radioactive.

(b). The RNA becomes Radioactive is the answer to this part of this question because CTP is needed in the experiment.

(c). The answer to this part (c) of this question is ''No'' because the only thing that takes part in bonding is the alpha phosphate.

(d). It depends. If the gamma phosphate and beta phosphate are not labeled, then the RNA will have certain degree of radioactivity.