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nata0808 [166]
3 years ago
5

Why are two different restriction enzymes used to cut the pUC19 plasmid and the lux gene DNA? What would have happened if only H

inD III enzyme was used
Biology
2 answers:
stealth61 [152]3 years ago
6 0
Two different restriction enzymes are used to cut the pUC19 plasmid and the lux gene DNA because there might not be one restriction site bordering the gene to be <span>because there might not be one restriction site bordering the gene to be. Hope this helps. Have a nice day.</span>
maksim [4K]3 years ago
4 0
Two different restriction enzymes are used because the restriction enzymes used must create two compatible ends. If only one enzyme is used blunt ends that are not compatible will be created.
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Gene flow and genetic drift both affect allele frequencies in a population. A high level of gene flow into a population genetic
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Proteins, large complex molecules, are major building blocks of all living organisms. Discuss the following in relation to prote
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Answer:

Proteins, large complex molecules, are major building blocks of all living organisms. Discuss the following in relation to proteins.

(a) The chemical composition and levels of structure of proteins.

Proteins are chemically macromolecules formed by manomeric units called amino acids. The structural organization of proteins is as follows:  Primary, Secondary, Tertiary and Quaternary.

(b) The roles of DNA and RNA in protein synthesis

From DNA, ribosomal RNA is formed, a type of RNA present in ribosomes that is responsible for protein synthesis. Therefore, the role of DNA in protein synthesis is essential: without DNA, there are no proteins.

(c) The roles of proteins in membrane structure and transport of molecules across the membrane

Proteins can work by transporting ions in different ways.

Explanation:

(a) The chemical composition and levels of structure of proteins.

Proteins are chemically macromolecules formed by manomeric units called amino acids, these have in their structure a carboxyl group and amino group, attached to the same carbon. To be assimilated by the body, proteins must be degraded in the amino acids that make them up.

The amino acids are linked together by peptide bonds. In those bonds, the amino group of one amino acid reacts with the carboxyl group of the other.

The structural organization of proteins is as follows:

Primary: Sequence of the amino acids in the chain with peptide bonds.

Secondary: Spatial arrangement of the amino acids of a protein. They stabilize by means of hydrogen bonds. There are two types: the propeller a and the folded blade b.

Tertiary: Three-dimensional arrangement of the polypeptide chain, stabilized by forces of Waals.

Quaternary: Union of weak bonds of arias polypeptic chains that originate a protein complex.

(b) The roles of DNA and RNA in protein synthesis

RNA fulfills numerous functions, the most important being protein synthesis, in which it copies the genetic order contained in the DNA to use it as a standard in the manufacture of proteins and enzymes and various substances necessary for the cell and the organism. For this, it goes to the ribosomes, which operate as a kind of molecular protein factory, and it does so following the pattern that the DNA prints on it.

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