Question

Why are two different restriction enzymes used to cut the pUC19 plasmid and the lux gene DNA? What would have happened if only HinD III enzyme was used

Answer 1

Two different restriction enzymes are used to cut the pUC19 plasmid and the lux gene DNA because there might not be one restriction site bordering the gene to be because there might not be one restriction site bordering the gene to be. Hope this helps. Have a nice day.

Answer 2

Two different restriction enzymes are used because the restriction enzymes used must create two compatible ends. If only one enzyme is used blunt ends that are not compatible will be created.