Answer:
DNA restriction enzymes cut the DNA molecule, while DNA ligases join the resulting DNA fragments
Explanation:
Transformation is a naturally occurring process by which bacteria incorporate exogenous genetic material from their surrounding environment. This process (transformation) is used for DNA cloning via plasmid vectors. In DNA cloning, transformation occurs after restriction enzymes cut the DNA at specific sequences named palindromic sequences (i.e, sequences that can be read the same in opposite direction). Restriction enzymes can generate sticky-ends, where enzymes make staggered cuts in the two strands (e.g., <em>BamH</em>), or blunt ends, where the resulting strands are of the same length (e.g., <em>HaeIII</em>). In general, sticky-end enzymes are more useful because they generate a 3' overhang in one molecule and a complementary 5' overhang in the other, increasing the yield and specificity of ligation. During ligation, a DNA ligase is used to join both DNA strands by forming phosphodiester bonds in the plasmid. Following transformation, bacteria can be selected on antibiotic plates.
<span>(3) parasite. Parasites are organisms that live on other organisms and cause harm to them.</span>
Below are the choices:
<span>a. creating an online chat group for asking questions and posting solutions
b. establishing a standard method for delivering the results to the teacher
c. offering a wide variety of rain gauges for everyone to choose from
d. using a standard unit of measure for the duration of the study
</span>
the answer is D. I hope it helps.
B) honey
A shirt is a solid, and helium is a gas at room temperature. A nebula is... not an object.
