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andrew-mc [135]
3 years ago
12

Normal cells die in a nutrient medium containing thymidine and methotrexate, whereas mutant cells defective in thymidylate synth

ase survive and grow. The mutant cells grow because the medium contains the___________ they are unable to make normal cells, however, continue to synthesize their own and thereby convert their limited supply of to________ . The methotrexate inhibits , so cannot be regenerated. Without a supply of___________ for the synthesis of nucleotides and amino acids, the cells die.
Biology
1 answer:
Dmitry_Shevchenko [17]3 years ago
7 0

Answer:

*Thymidine

*THF to DHF

*THF

Explanation:

Normal cells die in a nutrient medium containing thymidine and methotrexate, whereas mutant cells defective in thymidylate synthase survive and grow. The mutant cells grow because the medium contains the_____Thymidine______ they are unable to make normal cells, however, continue to synthesize their own and thereby convert their limited supply of to__THF to DHF______ . The methotrexate inhibits , so cannot be regenerated. Without a supply of______THF_____ for the synthesis of nucleotides and amino acids, the cells die.

NOTE:

THF means Tetrahydrofolate

DHF means Dihydrofolate

Thymidine which is also reffered to as deoxythymidine, is described as a deoxynucleoside that is composed of the pyrimidine base thymine that is attached to the sugar deoxyribose. Thymidine is a nucleoside made up of pentose sugar, pyrimidine base tymine and deoxyribose, it is one of the the four nucleoside found in exclusively DNA as well as T-loop of tRNA.

Tetrahydrofolate also known as tetrahydrofolic acid can be described as part of folic acid that function as a coenzyme which aid metabolic reactions that includes nucleic acid and amino acids. It is also reffered to as one-carbon metabolism, and it's oone-carbon mechanism is associated to the histidine, serine of amino acid.

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