Answer:
C.The added epitope disrupts the function of the tagged protein
Explanation:
When an additional sequence is tagged to a protein to use comercial antibodies, there are several reasons why this procedure wouldn't work as expected (note that we're assuming the protein is being expressed but it's not possible to detect it).
For example, the sequence of nucleotide added to codify for the tagged epitope are removed during the RNA processing. In that case, the protein would be expressed without the epitope, so it would be impossible to localize it with the antibodies.
Also, it could be that the new epitope is affecting some way the protein folding, making it not functional. This way, it would be degraded by the cell so it wouldn't be detected.
Another possibility is that the epitope doesn't affects the protein folding nor its function, but during the folding ends up in a conformation that makes it inaccesible for the antibody.
In summary, the way as the possible answers to this question are shown, the correct option seems to be C:<em> The added epitope disrupts the function of the target protein.</em>
Answer:
Xylem & Phloem are two types of vascular tissue found in plants.
Answer: positive feedback loops enhance or amplify changes. this tends to move a system away from its equilibrium state and make it more unstable. Negative feedbacks tend to dampen or buffer changes. this tends to hold a system to some equilibrium state making it more stable.
Explanation:
Answer: A diet rich in calcium and vitamin D supplements.
Some examples off calcium rich foods include Cheese; Yogurt; Sardines and Canned Salmon; Beans and Lentils; Almonds; Whey Protein; Some Leafy Green.
