Answer and Explanation:
The principal thing in life is vitality, without which not a solitary response in the body won't happen. In bioenergetics the responses of digestion i.e catabolism and anabolism will happen in both of the two different ways:
Endergonic reaction
furthermore, Exergonic reaction
In Endergonic reaction it will require vitality input while in Exergonic reaction will give out vitality as warmth/ATP. The significant type of vitality in the free vitality that is accessible type of vitality ∆G.
At the point when the reaction is endergonic ∆G is certain which indicates vitality in provided that is input and when the reaction is exergonic ∆G is negative meaning that vitality is given out. The magnificence of bioenergetics is the vast majority of the occasions it couples both of these response to get greatest vitality yield (exergonic). Most exergonic response will gives out vitality as ATP which is the vitality money of the cell utilized in the trading of response. Compounds are the impetus which consistently diminishes initiation vitality of the response so happen it suddenly i.e exothermically. T
Atoms of different elements always combine in the same way.
John Dalton noted that if the components are added to one exacerbated, the share of their masses will dependably be the same. Dalton's been an English scholar. The component mass of that mix is measured by the confirmation of the presence of molecules assembled by John Dalton.
Carbon dioxide fluctuations occur for different seasons of the year due to the presence of plants in an area.
<h3>Why carbon dioxide fluctuations occur for different seasons of the year?</h3>
The carbon dioxide levels in the atmosphere moves up and down every year when plants use carbondioxide gas through the process of photosynthesis and respiration. Plants take up the carbondioxide gas in the spring and summer season and release it in fall and winter season. In this modern world, the level of carbon cycle is increasing because more carbon dioxide is emitted from burning fossil fuels and other human activities.
So we can conclude that carbon dioxide fluctuations occur for different seasons of the year due to the presence of plants in an area.
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12is the speed of 6 seconds cause the bigger the secs the slower
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
