Even though you didn't give us the fragment with the comma slice, it isn't really necessary here as we can answer just based on these 4 options here. All options apart from the second one are correct - only the second option doesn't fix the mistake of the comma splice, whereas the other options do but connecting the sentences properly.
Answer: Dr. Seward had observed that the Renfield is a lucid.
Explanation:
Renfield used to collect flies and spiders. Steward had appointed a guard outside Renfield's cell to observe and regulate the aberrant behavior.
One night when Renfield screamed from his cell, Seward observed that he was badly hurt. His face was brutally beaten. He realized that Renfield cannot himself inflict wounds on his face, as his back was broken.
The script, or text of a play contains B. dialogue and stage directions.
The author writes stage directions so both the actors and the audience know what is going to happen and to set the stage better. A play without dialogues (or monologues) is not possible, is it?