Answer:
the diagram explains the process of DNA digestion and DNA ligation, which is usually used in molecular cloning techniques
Explanation:
Molecular cloning can be defined as the process used to synthesize multiple copies of a particular DNA fragment. Molecular cloning requires the insertion of a foreign DNA fragment into an appropriate vector (e.g., a plasmid) through the action of specific enzymes that serve to cut and ligate DNA fragments. DNA digestion and DNA ligation use specific restriction enzymes and DNA ligases, respectively, in order to insert the foreign DNA fragment. For this purpose, restriction enzymes that generate single-stranded overhangs are preferred to create sticky ends which bind by complementary base pairing. Subsequently, a DNA ligase enzyme joins the DNA fragments together in order to create recombinant DNA molecules. DNA Ligation is often achieved by using a specific T4 DNA ligase, while there are many restriction enzymes that generate sticky-ends (e.g., BamHI, EcoRI, BaI228I, etc).
Answer:
Promoter.
Explanation:
It is promoter because Promoter is a DNA sequence where's the gene transcription begins. It is the
DNA sequence which RNA polymerase binds or join to
so as to begin transcriptionn of a gene . It is a region where the regulatory elements i.e protein will bind to and the Promoter sequences are found directly upstream or at the end of 5' of the transcription initiation site. This can also encode RNA such as mRNA, trans and so on.
Answer:
Upon nutrient limitation, budding yeast will produce daughter cells less than 20% of the mother cell size. This asymmetric division may select for growth functions that are efficient over a larger range in cell sizes, such as exponential growth. In turn, efficient growth over a large size range lessens the pressure to have precise size control.
Explanation:
In wild-type cells growing in nitrogen-rich medium, the size threshold to enter mitosis is high, and the G1/S size control is cryptic because cell division produces daughter cells with a size greater than the minimum required to initiate S phase. In these conditions, G2 is long and G1 is short. However, the cell size threshold to enter mitosis is greatly reduced when wild-type cells are shifted to medium with a poor nitrogen source, such as minimal medium with proline, isoleucine, or phenylalanine. In these conditions, wild-type cells initiate mitosis at a reduced cell size, generating two daughter cells that are smaller than the critical size threshold required to progress through G1/S
Oil spill or a discharge from pipe from a factory or sewage system. Since this is a single point of origin water pollution, this type of water pollution furnish itself to relatively easy remediation.
What is point source water pollution?
Point-source water pollution is the one type of water pollution which is easy to recognize because it generates from a single source or event and affects a specific area.
The negative effects of single point origin is deteriorating water quality which damage the environment, health conditions.
Destruction of biodiversity is another negative effect of water pollution depletes aquatic ecosystems and prompt unbridled proliferation of phytoplankton in lakes called as eutrophication.
The solution to reduce water pollution is reduce carbon dioxide emissions to prevent global warming and acidification of the oceans. Another is to reduce the use of chemical pesticides and nutrients on crops.
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Answer:
The correct answer is Option B (Carrying out research). The step that follows this step involves forming B.a conclusion
Explanation:
The Natural environment around us is a function of both the living things and the non-living things. So, environmental science is the study of the natural environment around us. This study includes how to tackle environmental problems and further study on how humans could be impacted by the interactions with their environment. The scientific method is a step taken towards ensuring development in a field by bringing new discoveries and challenging existing beliefs through scientific tests and research. Generally, for most scientific methods of many fields, it starts with:
1. Observations
2. Making inquiry which involves asking questions
3. Forming a hypothesis, which means making untested assumptions
4. Conducting an experiment to check the hypothesis
5. Analyze the results
6. Publish the results
The correct answer is Option B (Carrying out research). Because it involves the scientific method highlighted above. The step that follows this step involves forming B.a conclusion