1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
madam [21]
3 years ago
14

An ampicillin-resistant, tetracycline-resistant plasmid, pBR322, is cleaved with PstI, which cleaves within the ampicillin resis

tance gene. The cut plasmid is ligated with PstI digested Drosophila DNA to prepare a genomic library, and the mixture is used to transform E. coli K12. Which Antibiotic should be added to the medium to select cells that have incorporated a plasmid
Biology
1 answer:
elixir [45]3 years ago
6 0

Answer:

tetracycline

Explanation:

A plasmid is a small, circular, double-stranded DNA molecule, which is physically separated from chromosomal DNA and is able to replicate independently. Moreover, plasmid transformation is the process by which exogenous DNA is introduced into a cell (usually a bacterial host cell). In this case, <em>Escherichia coli </em>K12 cells were transformed with the pBR322 plasmids that encode both ampicillin and tetracycline resistance genes. According to data, plasmids were firstly cleaved with the PstI type II restriction endonuclease within the ampicillin resistance gene, thereby these plasmids lose the ampicillin-resistance gene that allows selecting <em>E. coli </em>K12 cells by their resistance to ampicillin. In consequence, tetracycline is the only one selective marker that will indicate that the bacteria have incorporated the plasmid.

You might be interested in
What is the role of cyclin and CDK in the cell cycle? *
inessss [21]

Answer:

They perform checkpoints and regulate/control the cell cycle.

Explanation:

Cyclin/CDK complexes are known to regulate both mitotic and meiotic cell cycles. Some processes are shared by both forms of cell division, however the process known as meiosis differs in terms of its features and needs. Following two rounds of cell division in succession, meiosis is characterized by the replication of DNA.

5 0
3 years ago
Urban areas tend to be warmer than the rural areas that surround them. This is mainly caused by how the materials that humans us
Alona [7]
I think A is the answer
3 0
4 years ago
Read 2 more answers
A _____ IS CUT PART OF STEM WHICH IS PLACED ON A STOCK (ANOTHER ROOTED STEM)
Nitella [24]

Answer:

Explanation:

SCION is the answer.

Hope it helped you.

3 0
3 years ago
Read 2 more answers
Is fructose-1,6-bisphosphatase activated, inhibited, or unaffected in response to a glucagon signal?
Crazy boy [7]

Fructose-1, 6-bisphosphatase is activated in response to a glucagon signal.

<h3>What is the purpose of Glucagon signals?</h3>
  • Glucagon signals specify that fuel molecules are required in the bloodstream.
  • So these signals activate Fructose-1, 6-bisphosphatase (F1, 6BP) so as to increase the level of sugar and other fuels required for production of energy.
  • In the process of gluconeogenesis, new glucose molecules are synthesized from other non carbohydrate sources.
  • As glucose is required in the bloodstream so F-1, 6BP should be activated as it acts as a rate limiting enzyme in gluconeogenesis.
  • Gluconeogenesis occurs when the dietary intake of glucose is very low.
  • Glucose is required as a source of energy by almost all body cells so in case of less oxygen it is synthesized from other molecules like amino acids.

Learn more about gluconeogenesis here:

brainly.com/question/1425339

#SPJ4

7 0
2 years ago
If a fossil is 1/4 of the Carbon-14 it began with. How old is the fossil?
pishuonlain [190]

Answer:

b

Explanation:

half of the original c-14 isotope remain in the fossil 5730 years after the organisms died. 1/4 of the original isotope will remain 5730 years after that or 11460 years after the organism died.

4 0
3 years ago
Other questions:
  • What is the order for ABCD? (Convection currents) for example BCDA
    7·1 answer
  • A gardener stops a stem from becoming longer by cutting off the tip of
    8·1 answer
  • What is the approximate population of snowshoe hares in 1865?
    11·1 answer
  • What organisms have flagella?
    6·2 answers
  • PLEASE HELP! ASAP! FIFTEEN POINTS!
    11·1 answer
  • Question 16
    8·1 answer
  • Early British anthropological researchers believed they could isolate and scientifically study the structure and specific detail
    6·1 answer
  • How can an allele frequency
    14·1 answer
  • Diffusion ____ the cell.<br> A. eradicates<br> B. hinders<br> C. benefits
    13·1 answer
  • If the original strand reads ATT-CCC-GCA what is the complementary strand?
    15·2 answers
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!