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pychu [463]
3 years ago
9

What is a plasmid? How is a plasmid used in gene splicing?

Biology
2 answers:
Snowcat [4.5K]3 years ago
7 0
Plasmids are used in genetic engineering to reproduce RECOMBINANT genetic material. When a plasmid is inserted a bacterium, the bacterum is encouraged to multiply, creating more copies of the recombinant DNA.
julsineya [31]3 years ago
3 0
It is a circular piece of DNA that acts as a vector (a carrier of the gene). it attaches to the gene that is to be genetically engineered to form recombinant DNA (combined DNA of two different organisms). It allows the gene to be carried to a host cell to be replicated producing many identical genes. To cut open the plasmid requires an enzyme called restriction endonuclease and to stick the gene into the plasmid requires an enzyme called ligase
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Plz help i have attachment file
quester [9]

I believe that it is D. All of the above.

<em>If this is incorrect, please, do tell me and I can fix my mistake. Thank you.</em>

6 0
3 years ago
You determine that you have only three copies left of an important DNA fragment, so you decide to amplify it. Using flanking pri
kicyunya [14]

Hello. This question is incomplete. The full question is:

"You determine that you have only 3 copies left of an important DNA fragment, so you decide to amplify it. Using flanking primers, how many PCR cycles would you have to run to generate over one billion (10^9) copies of the fragment? "

Answer:

Approximately 29 cycles of PCR would be required.

Explanation:

As you may already know, PCR is a technique used in molecular biology that allows part of a DNA molecule to be recycled into millions of copies.

PCR allows this replication to be done through cycles. Each PCR cycle lasts about 2 minutes and allows the DNA molecule to undergo the separation of the strands, the binding of the primers and the synthesis of new DNA strands through DNA polymerase. This cycle is usually repeated 32 times, but this number may change depending on the number of copies the researcher thinks is necessary.

In this case, if a researcher wants 10 ^ 9 copies of DNA, he must do the following calculation to find the number of PCR cycles needed:

32 ^ x = 10 ^ 9

x = 28.3 = approximately 29 cycles.

4 0
4 years ago
Which of the males could be the father in the following DNA fingerprint?
lisov135 [29]

Answer:

I belive its either male 2 or both

Explanation:

4 0
3 years ago
The study of how people seek to satisfy their needs and wants by making choices is total cost
dsp73

Answer: I believe the correct answer is false.

Explanation:

4 0
2 years ago
How are Ionic bonds formed?
irinina [24]
Ionic bonding is the complete transfer of valence electron(s) between atoms. It is a type of chemical bond that generates two oppositely charged ions. In ionic bonds, the metal loses electrons to become a positively charged cation, whereas the nonmetal accepts those electrons to become a negatively charge

6 0
3 years ago
Read 2 more answers
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