Combining DNA from different sources is the definition of Recombinant DNA or rDNA. This is what is more commonly referred to as genetic engineering. The applications for this technology are vast, but very expensive. The use of Recombinant DNA has seen applications in agriculture where plants are given immunity to certain diseases or molds from another plant which is resistant or where human DNA is combined with forms of bacteria to produce a more natural insulin for people who are diabetic.
Answer: Domain.
Explanation: I'm glad that you asked this question. When it comes to to biology and the identification of species, domain is the highest rank that you can find in taxonomy. If you look at the taxonomy ranks, this is the order: Domain > Kingdom > Phylum > Class > Order > Family > Genus > Species.
That is why domain includes all of the rest, because in the pyramid, it is the one at the top.
Answer:
<em> A nick is created between G and A in GAATTC when using Eco R1 site while a nick is generated between G and G in GGATCC when Bam H1 is used. The difference in the nicks created is the main reason you observe two bands when you digest the circular plasmid with a mixture of both</em>
Explanation:
Circular plasmid containing : 9 kb of DNA
Eco R1 and Bam H1 are known as restriction sites in DNA ( i.e. special sequences found in a DNA ) hence they can be specifically cleaved by the restriction enzymes enabling the insertion of a gene. that is why you will get a same result when you digest the Plasmid with either Eco R1 or Bam H1
<em>but A nick is created between G and A in GAATTC when using Eco R1 site while a nick is generated between G and G in GGATCC when Bam H1 is used. The difference in the nicks created is the main reason you observe two bands when you digest the circular plasmid with a mixture of both</em>
Answer: Light and Temperature
Explanation:
With more light, the photosynthesis occurs faster. In most cases the grower will try to have the photosynthesis occur as fast as possible. With a lot of light available, the temperature may go up. On darker days, a lower temperature is desired. Only with really high amounts and really low amounts, light can be influenced; respectively screening and supplemental lighting.
