If one wishes to clone a gene using typical restriction endonucleases, how does the restriction endonuclease identify the appropriate cut sites in the genome using the palindromic sequence.
Restriction endonucleases are a family of enzymes usually found in bacteria that cuts DNA at a specific sequence called recognition site. There are three type of restriction endonuclease Type I, Type II, and Type III.
Each endonuclease recognizes a particular sequence called palindromic sequence which is a sequence that reads the same sequence in both the strands either reading from the 3 prime end or 5 prime end.
The endonuclease cuts the palindromic sequence in such a way that it produces sticky ends.
To learn more about restriction endonucleases here
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The answer is; opioids
These painkillers relieve pain by competing with neurotransmitters in binding neurotransmitter receptors in the synapse. However, they don’t induce an impulse hence block the receptors from neurotransmitters. This working principle, however, is also problematic because it causes slow breathing because the drugs calm nerve activity even that which controls your breathing.
Answer:
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Explanation:
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