Bivariate cell cycle assay (BrdU/PI) using cell cycle-specific antibodies and DNA dyes can distinguish G0 from G1 phase or G2 from M phase
https://www.creative-bioarray.com/bivariate-cell-cycle-assay-brdu-pi.htm
Answer:
Transcription
Explanation:
This occurs in three steps itself: initiation, elongation, and termination
Initiation (beginning)
- RNA polymers bind to a promoter on a gene
- DNA then unwinds to be "read" by an enzyme
- DNA now ready to make mRNA strand
Elongation (Second step)
- nucleotides are added to mRNA strand
- mRNA molecule is built after RNA polymers reads the DNA that unwound
- adenine (A) in DNA connects to a uracil (U) of RNA while connected to each other
Termination (End)
- RNA will cross a stop sequence in the gene (termination)
- mRNA done and disconnects or detaches from DNA
Hope this helps!
Isopropyl alcohol causes the DNA to precipitate. When DNA is emerged in the solution, when it comes out it will ideally clump together, making it visible. Isopropyl alcohol is a key ingredient when isolating DNA. Ethanol also can be used to achieve this.
A, B, and D hope it helps