Hi
Below are five major steps of DNA or gene cloning:
1: You chose the gene or specific piece of DNA you need to clone and cut the gene with restriction enzymes from the source organism.
2: You need to choose a vector for the process of cloning and you will cut the vector with same restriction enzymes through which you have cut your target DNA sequence to be inserted into the vector.
3: You place the target gene into the vector and join or seal the gene with vector by using an enzyme called DNA ligase.
4: You introduce the vector with your target gene into a suitable host organism such as yeast or bacteria through the process of Transformation. In this process host organism takes up the vector containing your target gene and starts replicating the target DNA along with their own DNA and thus creating millions of copies of target gene .
5: In the last step, the DNA or target gene is isolated from host organism and purified and is ready to be used since its quantity has been enormously increased through the process of cloning.
The cloning is also called as recombinant DNA technology and is the main process that is being used in the production of insulin for diabetes patients. You can see below image for better understanding.
Hope it help!
Answer
The correct option is B.
Explanation
Ecological succession of any species is composed of following stages:
A. Migration
The movement of species from one place to another location for the purpose of setting permanently o temporary is known as migration
. Forexample
Siberian crane (<em>Leucogeranus leucogeranus</em>) that live in the arctic tundra of eastern and western Russia. The eastern population of birds migrate to china while western population of these birds migrate to Iran, India and Nepal during winter season.
B. Ecesis
The complete establishment of species in new habitat that was completely barren or left barren due to some catastrophe is known as Ecesion. This process is dependent upon the climatic, edaphic and biotic factors. The success of plant depend upon some of the adaptations to withstand the unfavorable conditions which includes both biotic and abiotic conditions.
Forexample
The process of succession in xerosphere start on barren area from pioneer stage covered by crustose lichen, then foliose lichen followed by moss stage, herbaceous stage and a last by shrub stage.
C. Nudation
The initiation of new species by major environmental disturbance such as volcano eruption is known as Nudation.
D. Reaction
It represents the effect of established species on the habitat. Forexample plants alter habitat condition as they grow. They extract raw materials from the environment in large amounts and return metabolic wastes. The waste accumulates in large amounts and differ from the original raw materials, thus altering the environment.
E. Competition
The phenomenon which involves struggle for existence between two or more individuals growing in same area, that make excessive demand that are similar in nature on the soil is known as competition.
Such struggle may be in between same species or different species. The competition may be interspecific or intraspecific. Because of completion the weak individuals are eliminated as strong ones are retained.
Detrital<span> or clastic </span>sedimentary rocks<span> are composed of</span>rock<span> fragments. They are different than </span>chemical sedimentary rocks<span>, which are composed of mineral crystals. Learn how these </span>sedimentary rocks<span> differ in their formation and composition.</span>
They pollinate plants, which saves farmers work. Insects such as bees and butterflies help to pollinate plants.
