The DNA replication products visualized during the sanger method of DNA sequencing are observed in which nucleotides are added.
Sanger sequencing is based on the process of DNA replication. A scientist creates a copy of his DNA strand. Then observe which nucleotides have been added. This way you can see the sequence of nucleotides. A laser excites the fluorescent labels in each band and a computer detects the resulting light.
Sanger sequencing produces extension products of various lengths ending in dideoxynucleotides at the 3' ends. Extension products are separated by capillary electrophoresis or CE. Molecules are injected by an electric current into a long glass capillary filled with gel polymer. Selective incorporation of chain-terminating dideoxynucleotides by DNA polymerases during in vitro DNA replication.
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1) 5 kingdom system of classification is a type of classification in which all the living organisms are classified into 5 kingdoms i. e. protista, monera, fungi, plantae and animalia.
2) Plants are autotrophic organisms while animals are heterotrophic. Plants have cell wall while cell wall is absent in animal. Plants are fixed while animals move from place to place.
3) Both plants and animals are made of cell. Both plants and animals are multicellular organisms. Both plants and animals reproduce.
4) Plants make their own food while fungi are unable to make their own food. Plant cell wall is made of cellulose while fungi cell wall is made of chitin.
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a. Midwest has few sources of acid rain-forming pollutants.
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Midwest has few sources of acid rain-forming pollutants because Midwest has less number of factories which produces less pollution. Farming, mining, and manufacturing of different products are the major industries Midwest. Other industries like transportation, finance, and machinery are also important for the economy of Midwest. Due to less number of factories less amount of pollutants goes to the atmosphere and will not cause acid rain.