Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Answer:
in my opinion lol
Explanation:
Why : bc our mind is still active even while we r sleeping i think
What: to imagine things that will never happen but what we want.
A A because it look like the definition
1) Fine flour and lumpy flour - B) Sieving
2) Mixed nuts - A) Handpicking
3) Sand and gravel - B) Sieving
4) Sand and palay - B) Sieving
5) Scrap iron and nail - C) Using magnet
(mark me brainliest if you're satisfied with my answer :))
Some molecular studies point to amphibians descending from the lung fish, the Diponi.
Under the class Amphibia, amphibians are tetra-pod, cold blooded vertebrates without having scales. Protein is produced by the special skin of Amphibians.
Lung fish belong to the subclass of Diponi, these are living air breathing species.
