The process of fusing two or more DNA molecules to produce a hybrid is known as recombinant DNA. Restrictions endonucleases and ligases are two classes of enzymes that enable the technique.
When a restriction endonuclease detects a particular DNA sequence, it makes cuts inside or near that sequence. The recognition sequence of a restriction enzyme will haphazardly appear on every (1–4)n bases along a random DNA chain. The equation states how many fragments ends (measured in moles) are produced when a restriction enzyme cuts DNA.
Moles of DNA ends =2x(grams of DNA)/(number of bp)(660 g/mol / bp).
The equation describes how many ends are produced when circular DNA is digested by a restriction enzyme.
Mole ends=2x(moles DNA)x number of restriction sites.
The following equation can be used to determine how many ends are produced during the digestion of a linear molecule by a restriction endonuclease.
Mole ends=2x(molesDNA)number of restriction sites +[2x(moles of DNA)].
learn more about DNA brainly.com/question/2131506
#SPJ4
Answer:
In the entire solar system, the environment of planet earth is unique due to the presence of the atmosphere.
It is responsible for providing carbon dioxide and oxygen which are essential for the presence of life on earth.
Secondly, it helps in keeping the earth warm through the greenhouse effect.
It prevents the entry of harmful radiations of the sun into the atmosphere.
Answer: ionotropic; metabotropic
Explanation:
A receptor can directly open the channel and exerts an ionotropic effect. The ionotropic effect takes place by the help of ionotropic receptors. These receptors are membrane bound receptor proteins which responds by the bonding of the ligand.
Due to ligand binding the channel opens and allows the movement of ions into the cells which helps in either increasing or decreasing the action potential.
The receptors can also bind to the ligand and produce metabotropic effect which means by the second messenger.
Smooth muscles- protects the organs
Cardiac muscle- around the heart
And skeletal
