Answer:
1-by transfecting small interfering RNAs against target genes of interest
2-by using comparative genomics strategies in order to infer functional relationships among target genes of interest and homologous genes responsible for virulence in other bacteria (e.g., antibiotic resistance genes)
3- by inducing mutations through site-directed mutagenesis in order to study gene function (i.e., by examining the effects of knockout mutations)
Explanation:
Whole-genome sequencing (WGS), also known as complete genome sequencing, refers to Next Generation Sequencing technologies that allow the obtention of the entire genetic sequence of an organism/cell, which can be used as a reference genome to understand gene function, evolutionary relationships, etc. The information provided by WGS technologies allows making many different types of genetic analyses in order to understand gene function. First, the nucleotide reference sequence can be used to design complementary small interfering RNAs that trigger degradation of target messenger RNAs (mRNAs) by the RNA interference (RNAi) pathway, thereby inhibiting gene function (in this case, inhibiting genes associated with virulence in the bacterial strain). Second, a reference genome is required to perform bioinformatic data analyses in order to identify homologous genes associated with virulence in evolutionarily related bacteria, allowing identify, for example, antibiotic resistance genes or sequence polymorphisms (e.g., single nucleotide polymorphisms, SNPs) associated with gene function. Third, the information provided by a reference genome can also be used to trigger site-directed mutagenesis (for example, by using the highly precise CRISPR-Cas9 genome editing technology) in order to knock out specific genes of interest and thus analyze if the bacterial strain is still infectious.
With one strike of lightning there is almost no chance you will survive
Answer:
14 CO₂ will be released in the second turn of the cycle
Explanation:
<u>Complete question goes like this</u>, "<em>The CO2 produced in one round of the citric acid cycle does not originate in the acetyl carbons that entered that round. If acetyl-CoA is labeled with 14C at the carbonyl carbon, how many rounds of the cycle are required before 14CO2 is released?</em>"
<u>The answer to this is</u>;
- The labeled Acetyl of Acetyl-CoA becomes the terminal carbon (C4) of succinyl-CoA (which becomes succinate that is a symmetrical four carbon diprotic dicarboxylic acid from alpha-ketoglutarate).
- Succinate converts into fumarate. Fumarate converts into malate, and malate converts into oxaloacetate. Because succinate is symmetrical, the oxaloacetate can have the label at C1 or C4.
- When these condense with acetyl-CoA to begin the second round of the cycle, both of these carbons are discharged as CO2 during the isocitrate dehydrogenase and alpha-ketoglutarate dehydrogenase reactions (formation of alpha-ketoglutarate and succinyl-CoA respectively).
Hence, 14 CO₂ will be released in the second turn of the cycle.
Infants’ bodies contain more water (75%) than those of older humans. Their kidneys are not fully mature yet thus they excrete more diluted urine. This cause easy dehydration and a higher risk of illness for infants. Infants are less resistant to heat since their thermoregulatory systems are not as developed. Parents and caregivers must see to it that infants are provided water for their hydration.
Decomposers are organisms that break down dead organisms and release nitrogen back into the cycle
