Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
<span>The nurse will most likely observe Paget's disease on the client's medical chart. Paget's disease interferes with the body's bone tissue recycling process and results in increased blood flow around affected bones, flushing extra alkaline phosphatase and urinary hydroxyproline from the body.</span>
1. C
2.B
3.C
4. A
5. False
6.True
7.False
8.True
9.True
10. false
Hopefully this help
<u>Answer:</u> The weight of the person above the surface of a planet is 635.83N.
<u>Explanation:</u>
To calculate the weight of a person, we use the formula:
....(1)
where,
w = weight of an object
m = mass of the person = 65kg
g = acceleration due to the gravity of the planet
For the calculation of weight, we need to first find the acceleration due to gravity and for that we use the formula:
where, g = acceleration due to gravity = 
G = Universal gravitational constant = 
M = mass of the planet = 
r = distance of the person from the planet = 
Putting values in above equation, we get:
Putting this value in equation 1, we get:
Hence, the weight of the person above the surface of a planet is 635.83N.
