Frozen red blood cells that have been thawed, deglycerolized and reconstituted in an open system must be used within 24 hours.
Cryopreservation of Red blood cells (RBCs) can be done with a long time span of usability of 10 years. Glycerol safeguards RBCs during freezing and defrosting, yet it can cause hemolysis if it is not washed off or eliminated before transfusion. When a unit has been deglycerolized, the shelf life of RBCs in an open system is only 24 hours, bringing about sporadic utilization of Frozen RBCs from the outset of the procedure or in close system cases it is up to 14 days.
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Boyle's law relates pressure and volume
Answer: Hydrogen bonds are created between the base pairs in DNA. Adenine pairs with thymine and cytosine pairs with guanine. Adenine and thymine create 2 hydrogen bonds while cytosine and guanine create 3. These bonds are fairly weak so that they can be broken when DNA is replicated (by an enzyme called helicase which unwinds DNA). However despite them being weak they do stabilize DNA's double helix structure as well.
