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GuDViN [60]
3 years ago
11

Bacteria are a commonly used organism for studies of genetic material in the research laboratory. The nucleic acids must first b

e isolated from the cells for these studies. Which of the following would most likely be used to lyse the bacterial cells for nucleic acid isolation?
Biology
1 answer:
BartSMP [9]3 years ago
6 0

Answer:

The options are not given but the most probable answer is - Lysozyme

Explanation:

Bacteria are commonly used in many genetic research program. So for genetic research, the nucleic acid of bacteria has to be taken out for the molecular studies.  

So to take out the genetic material of bacteria first their cell wall has to be broken and destroyed. Lysozyme is the enzyme that targets the peptidoglycan layer in the bacterial cell wall and breaks it which causes cell wall lysis in bacteria.  

So lysozyme is the enzyme which can be used to lyse the bacterial cells for nucleic acid isolation.

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How does mass extinction affect species that survive?
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What is the biochemical explanation for why adding glucose to a bacterial culture containing lactose significantly decreases pro
MatroZZZ [7]

Answer:

If we add glucose to the culture with lactose, the <em>lac operon</em> would be turned off and the production of <em>beta-galactosidase, </em>other enzymes and molecules that the operon transcribes, would decrease.

On the contrary, if we add lactose to a bacterial culture containing glucose, the <em>lac operon</em> would be on, transcribing the genes, hence, producing beta-galactosidase but only at a small rate. Because the bacteria already got the energy from the glucose.

Explanation:

An operon is a group of structural genes whose expression is regulated by a <em>promoter, operator and regulator genes</em>. In this particular case, they are talking about the lactose operon, <em>lac operon</em>. This operon is required, in bacteria, to obtain energy from lactose when there is no glucose present.

The lac operon contains a series of genes, <em>lacZ, lacY</em>, and <em>lacA</em>. The gene <em>LacZ</em> codifies an enzyme required to break the lactose, <u>beta-galactosidase</u>. <em>LacY</em> codifies for another enzyme required in the transport of lactose inward the cell. And finally, <em>LacA</em> produces a molecule that is involved in detoxification.

 

The <em>lac operon</em> is regulated, among others, by the presence of glucose and lactose. The glucose is the preferable source of energy for bacteria so if there are lactose and glucose the glucose would be used for energy at a bigger extent. In the <u>absence of glucose</u>, the <em>lac operon</em> plays a vital role and helps the bacteria to obtain energy from lactose.

When there is glucose but no lactose available, there is a <u>repressor</u> than binds to the promoter so the <em>lac operon</em> is OFF cannot express the genes.

On the other hand,when there is lactose and no glucose, lactose enters the cell and some of this molecules turn to alolactose. <u>Alolactose molecules</u> bind to the repressor so the operon is not repressed anymore and the <em>lac operon</em> would be ON producing enzimes and molecules.

If the <em>lac operon</em> is ON ( glucose absent ) the production of <u>beta-galactosidase</u> would be high, on the other hand, if the operon is OFF (glucose present) the production of <u>beta-galactosidase</u> decreases.

In the presence of <u>both lactose and glucose</u>, the genes for the lactose metabolisms are transcribed at low rates in the <em>lac operon</em>.

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