1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
natima [27]
3 years ago
13

HELP PLEASE I GIVE YOU BRAINLIEST I NEED HELP WITH 2

Biology
1 answer:
Ivan3 years ago
3 0

Answer:

C. makes the most sense so I hope I'm right

Explanation:

If this isn't correct I am so so so sorry!

You might be interested in
What are the two major parts of the cell?
Setler [38]
I would say teh cytoplasm and nucleus.
3 0
3 years ago
Design an experiment to answer the question, what is the effect of caffeine on the rate of mitosis in frog eggs? Label: hypothes
s2008m [1.1K]

Answer:

Explanation:

Null Hypothesis: Their will be no effect of the caffeine on the mitosis.

Hypothesis: Caffeine will inhibit the SHSY5Y cell division.

Dependent variable: Various concentration of the caffein compound.

Independent Variable: temperatures of the experimental condition. If their will be any effect of the temperature in mitosis then it will also affect the mitosis of control cells.

Experimental design.

Culture SHSY5Y neuronal cell line in the T-75 culture flask in CO2 incubator at 37 degree celsius. Next day, add cells from T-75 flask in the 24 well plate and allow cells to adhere to the well by incubating plate in CO2 incubator at 37 degree celsius. Add the compound caffeine at different concentration (say 1 microgram, 2 microgram, 3 microgram, 4 microgram) and incubate the plate for 24 hour. Determine the mitotic cells by following assay.

Assay to determine mitotic cell.

The reagent succinimidyl ester of carboxyfluorescein diacetate (CFSE) binds to cytoplasmic proteins. it is fluroscent reagent. And when cell divide due to mitosis, cytoplasm will also divide and hence, the protein which stained with the CFSE reagent will also goes in the daughter cell. Then, the fluorescence of the stained cells can be read on the plate reader.

Prepare the graph of the fluorescence values (Y-axis) verses different concentrations of the caffein (X-axis) and determine the slop of curve to know the rate of mitosis.<u> </u>

<u>Following test and control samples will be used for the assay.</u>

1. SHSY5Y cell line with added caffeine. It is test sample.

2. SHSY5Y cell line added with buffer. This important to know behaviour of cells under experimental conditions.

3. SHSY5Y cell line with solvent used to dissolve compound caffeine. It is important to know the toxicity or any effect of solvent on the mitosis.

5 0
2 years ago
NEED HELP ASAP WILL GIVE BRAINLIEST THANK YOU
slamgirl [31]

Answer:

b

Explanation:

5 0
3 years ago
Read 2 more answers
The combining form​ cancell/o-, as in cancellous bone​ structure, means:
sattari [20]
The combining form cancel/o-, as in cancellous bones structures means: lattice structure.
5 0
3 years ago
Which of the following statements is true?
mezya [45]
4 is true with question you asked
7 0
3 years ago
Other questions:
  • Which type of lipid store extra energy and is liquid at room temperature
    7·2 answers
  • . Meditation and hypnosis are similar in that they both involve:. a. delta brain wave activity. . b. a stream of mental activity
    14·1 answer
  • Examples of different ways that observations can be used in scientific inquiry
    11·1 answer
  • What are 3 newton laws of motion?
    14·1 answer
  • What are the pros and cons of the paleontology protocol?
    7·1 answer
  • This nutrient is cycled between living things and the soil. Plants absorb this nutrient from the soil and turn it into organic c
    10·2 answers
  • A male flying tree squirrel with a medium-length tail has babies with a female with a medium-length tail. Over the years their t
    12·1 answer
  • 3. If tall eyeballs (T) are dominant to short eyeballs(t),
    14·1 answer
  • Is there a relationship between earth's different climates?
    9·1 answer
  • In desert areas covered with black rock from ancient lava flows, rock pocket mice with darker fur are common. In other areas of
    12·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!