Roche/454 pyrosequencing , useful for high throughput sequencing techniques. This method utilizes pyrosequncing which allows for sequencing as the sequence read out can be achieved at the same time as the sequence is extended.
High- throughput screening is the use of automated equipment to rapidly test thousand to millions of samples for the biological activities at the model organism, cellular, pathway or molecular level.Many DNA samples can be sequenced simultaneously.
Sanger DNA sequencing also known as chain termination. This method is used only for the single DNA fragment at a time. Amplicon sequencing with NGS is efficient and cost effective . It is required a DNA polymerase , nucleotide , dideoxynucleotides and buffer.
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I believe the answer is this:
DNA replication is called semi-conservative because half of the original double-helix appears in one daughter strand formed in replication if you need any more help then please comment and let me know ok. :)
The structure of plasma membrane makes it selectively permeable allowing it to regulate the passage of substances into and out of the cell. Small nonpolar molecules can easily across the phospholipid bilayer of plasma membrane because it is hydrophobic. Polar molecules and ion cannot easily across the hydrophobic portion of plasma membrane because it is hydrophilic. Polar molecules and ions usually across the plasma membrane with the help of transport proteins.