Seymour Benzer used phage T4 mutants, Experimental
evidence that a gene is a linear sequence of nucleotide pairs. Some regions of
chromosomes have "hot spots" for mutations. Benzer's experimental
approach to fine structure mapping of the rII locus. Generated 1612 spontaneous point mutations
and several deletions in rII locus identified two complementation groups, rIIA
and rIIB. Mapped locations of deletions
relative to each other using recombination.
Mapped locations of point mutations relative to the deletions. Tested
for recombination between all point mutations within the same complementation
group.
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<span>They are not visible because the chromatin coils up,shortens,and thickens.</span>
