Answer: For detection and confirmation of HIV antibodies in blood samples.
Explanation: As the name implies ELISA( Enzyme-linked immunosorbent assay) is the first test widely used for determining the presence of HIV in a person's serum because of its high sensitivity. In an ELISA, a person's serum is diluted 400 times and applied to a plate to which HIV antigens are attached. If antibodies to HIV are present in the serum, they may bind to these HIV antigens. The plate is then washed to remove all other components of the serum. A specially prepared "secondary antibody" (an antibody that binds to other antibodies) is then applied to the plate, followed by another wash. This secondary antibody is chemically linked in advance to an enzyme.
Thus, the plate will contain enzyme in proportion to the amount of secondary antibody bound to the plate. A substrate for the enzyme is applied, and catalysis by the enzyme leads to a change in color or fluorescence. ELISA results are reported as a number; the most controversial aspect of this test is determining the "cut-off" point between a positive and a negative result. A cut-off point may be determined by comparing it with a known standard. Unknown samples that generate a stronger signal than the known or control sample or are called "positive" while those that generate weaker signal are "negative".
Answer:
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Explanation:
Answer:
Fatigue and blurred vision
Explanation:
Diabetes -
It refers to as the metabolic disorder , where the sugar level in the blood is increased which hampers the insulin secretion in the body .
<u>The most common symptom of diabetes are as follows - </u>
- slow-healing wounds and cuts
Hence , from the given scenario of the question ,
The correct answer is Fatigue and blurred vision .
C should be the answer because in that example he is not prepared nor is his flexible easily flexible by him being out at the movies on a weekend that he is on call. It shows the importance of needing to be flexible and easily adaptable in case of emergency.