Given what we know, after you’ve removed a loopful of broth culture from the culture tube you should immediately apply a flame to the open end of the test tube.
<h3>Why would this be the next step?</h3>
Once you have removed the loopful of broth culture from the tube, you should apply a flame to the end of the tube, this is of vital importance. The reason for this is to deny any other contaminants from entering or exiting the culture sample.
Therefore, we can confirm that after you’ve removed a loopful of broth culture from the culture tube you should immediately apply a flame to the open end of the test tube.
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All the deserts are found at 30<span>°N because
</span><span>Hot air from the equator rises, then cools, moisture is lost, and the dry air comes down at 30 degrees north and south</span>
The answer to your question is B- introduction of non-native animal species
Some species we see in Alaska and nowhere else in the Wolrd are:
Bats
Shrews
Bears
Seals
White Wolfs
Sea lions
Racoons
Walrus.
Bears, Seals, White Wolfs and Sea lions survive the cold weather much more than those other animals.