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zheka24 [161]
3 years ago
14

One of your lab partners has followed the recommended procedure of running Gram-positive and Gram-negative control organisms on

her Gram stain of unknown species. Her choices of controls were E. coli and B. subtilis. She tries several times and each time concludes she is decolorizing too long because both controls have pink cells (one more than the other). What might you suggest she try and why?
Biology
1 answer:
kow [346]3 years ago
5 0

Answer:

The correct answer is "I would suggest her to change the Gram positive control from Bacillus subtilis to other bacteria".

Explanation:

Bacillus subtilis, and the species from the genre Bacillus, are known for losing the crystal violet coloration during Gram testing. It is likely that B. subtilis might look pink under the procedure for this reason. I would suggest to change her Gram positive control from B. subtilis to other bacteria. For instance, she could try with Staphylococcus aureus, a widely used bacteria as Gram positive control.

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The changing quantities in an experiment are called variables. A variable refers to any trait, factor, or a condition, which can prevail in distinct amounts or kinds. An experiment generally exhibits three kinds of variables: independent, dependent, and controlled.

The independent variable refers to the one, which is modified. On the other hand, the dependent variable is implied to observe that how it reacts with the change made to the independent variable. The controlled variables refer to the quantities, which one wants to remain constant.

In the given case, that is, heating a cup of water to witness that whether the sugar will get more easily dissolve. In this, the independent variable refers to the temperature of the water determined in degrees Centigrade. The dependent variable refers to the amount of sugar, which dissolves completely measured in grams.

In this, the controlled variables are stirring and type of sugar. As more stirring might elevate the amount of sugar that dissolves, and different kind of sugars might dissolve in distinct concentrations. So, in order to make sure a fair test, there is a need to keep these variables similar for each cup of water.


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