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zheka24 [161]
3 years ago
14

One of your lab partners has followed the recommended procedure of running Gram-positive and Gram-negative control organisms on

her Gram stain of unknown species. Her choices of controls were E. coli and B. subtilis. She tries several times and each time concludes she is decolorizing too long because both controls have pink cells (one more than the other). What might you suggest she try and why?
Biology
1 answer:
kow [346]3 years ago
5 0

Answer:

The correct answer is "I would suggest her to change the Gram positive control from Bacillus subtilis to other bacteria".

Explanation:

Bacillus subtilis, and the species from the genre Bacillus, are known for losing the crystal violet coloration during Gram testing. It is likely that B. subtilis might look pink under the procedure for this reason. I would suggest to change her Gram positive control from B. subtilis to other bacteria. For instance, she could try with Staphylococcus aureus, a widely used bacteria as Gram positive control.

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A usable dilution of disinfectant is one that prevents growth of bacteria when compared with the positive control tube. reduces
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When compared to the positive control, a disinfectant dilution is considered to be useful if it stops bacterial growth.

Choosing a disinfectant:

When selecting a disinfectant, keep the following things in mind:

  • The range of bacteria that need to be eliminated. Look into the specifications of the manufacturers.
  • The conditions of usage of disinfectant.

Application of disinfectants :

  • Activity: it is necessary for efficient disinfection since, once diluted, it degrades over time. Different disinfectants deteriorate at different rates. Obtain data from the manufacturers. Disinfectants that don't have a color indication, like Virkon, should be marked with an expiration date.
  • Touch time: it has to be verified that the manufacturer's data to make sure the organism stays in contact for the specified amount of time. Considering that solid materials like cells and tissue granules are difficult for disinfectants to penetrate, autoclaving would be a more effective way to inactivate.

Therefore the correct choice is an option (A).

Learn more about disinfectants here:

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2 years ago
In photosynthesis, water undergoes ______ while carbon dioxide undergoes ________
Dovator [93]
A,
oxidation is when a molecule loses electrons.
<span>in this case, water is losing an electron and CO_2 is gaining an electron, which then makes it an energy rich sugar molecule</span>
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How mutation cause colorectal cancer​
Veseljchak [2.6K]

Answer:

  • A very small portion of colorectal cancers are caused by inherited gene mutations. Many of these DNA changes and their effects on the growth of cells are now known. For example: Familial adenomatous polyposis (FAP), attenuated FAP (AFAP), and Gardner syndrome are caused by inherited changes in the APC gene.
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What letter represents the biological level for community?
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If a 100-pound person played tug of war with a 50-pound dog , how light difference forces affect the results?
Anika [276]

Answer: APPARENTLY YOU CAN battle a lion at the San Antonio Zoo. OK, it's not that kind of battle—it's a tug of war battle. There is a thick rope passing through a hole. On one end, there is a lion cub. On the other end of the rope there could be three professional WWE wrestlers. Who wins? Well, the wrestlers don't win. But there is also some physics here.

It's all about friction

A typical tug of war isn't really about strength—it's about friction. It doesn't matter how strong you are if you don't have enough friction to keep yourself from sliding. Let me show you. Here is a force diagram for two people in a tug of war.

Each puller (person pulling on the rope) has essentially four forces acting on them. There is the downward weight which is balanced by the upward push of the ground. Since the person pulls on the rope, the rope pulls on the person in the opposite direction (this because of the two-ended nature of forces). Finally, there is the frictional force between the person and the ground. If no one is "winning," all these forces add up to zero and the person stays at rest. In order to start moving in the winning direction, the frictional force must be greater than the force from the rope.

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This "two-ended" nature of forces means that forces come in pairs and are an interaction between two objects. It doesn't matter how hard the person on the left pulls on the rope, this same rope pulls on that person. So if you were to increase your pull to 1,000 Newtons—that same rope pulls on both people. So the key is the other force acting in the horizontal direction: friction.

The maximum frictional force depends on two things. It depends on the types of surfaces interacting (maybe it's the rubber on a shoe interacting with a concrete floor) where some surfaces just have more friction than others. You really can't change this part in a tug of war. The friction force also depends on the normal force. The normal force is the force with which the two surfaces are pushed together. In the case of this simple tug of war above, the ground pushes up with a force equal to the gravitational weight of the person. This means that heavier people will have a greater frictional force acting to help win a tug of war.

Oh, note that in the human vs. lion tug of war the humans are on a different surface than the lion. Who has the advantage? It's probably the lion since she could have uneven ground that would allow her to use more than just plain old sliding friction.

So, it's really about mass

If you have two contestants on equal ground with similar friction materials, mass matters. If the person on the left has half the mass of the person on the right, they will have half the friction. This means that it's pretty tough to beat a heavier puller.

What about the lion? This looks like a young lion—she could have a mass of about 120 kg (up to maybe 182 kg). A typical massive human wrestler might have a mass of around 90 to 100 kg. Three humans will probably have more mass than a single female lion. Probably. The humans should have an advantage in the mass.

It's also about rope friction

Wait! This isn't a normal tug of war. The rope isn't straight. Did you notice that? This can make a big difference in that it can add another frictional force into play. Let me show you how this works with a diagram of the rope as seen from the top where it passes through the glass.

Explanation:

3 0
3 years ago
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