Question

Under normal cellular conditions, the FoF1 complex of the inner mitochondrial membrane functions to create new ATP. Under non-physiologic experimental conditions, isolated FoF1 complex can be driven in reverse as long as [ATP] is high. Which of the following would be true under such conditions?
A. The FoF1 complex would be operating as an ATPase rather than an ATP synthase.
B. The FoF1 complex would be driving net H+ movement from the matrix side to the IMS (intermembrane space) side
C. The FoF1 complex would create more ATP, per unit time, than it does under physiological conditions
D. As the experiment continues, we would expect to see higher & higher concentrations of ADP.
E. The FoF1 complex would effectively be operating as a proton pump.

Answer 1

Answer:

The answer is "Option A,  B, and E".

Explanation:

In specific cell conditions, the F0F1 system of the inner mitochondrial membrane functions as an ATP synthase for generating new ATP ADP proteins and Pi. Even so, this has a specific biological role in non-physiological situations through functioning as an ATPase. Its ATPase acts in such circumstances and transmits large amounts of ATP to both the mitochondrial space throughout the transmembrane proton gradient.

The free radical of the transmembrane can be used by fermenting bacteria without an electron transport system. Its ATPase is being used to generate a proton gradient rather than to hydrolyze ATP. It may be used as a means of transporting flagella or nutrients into its cell.