Answer: Tetracycline.
Explanation:
A plasmid is a small circular DNA molecule found in bacteria that is separate from the bacterial chromosome and replicates independently of it. They encode for certain genes that play a key role in antibiotic resistance. <u>Restriction enzymes are endonucleases that catalyze the cleavage of phosphodiester bonds in different regions located within a DNA strand</u>. PstI is an example of an endonuclease, it is a type II restriction enzyme produced by the microorganism <em>Providencia stuartii</em> that possesses a restriction target in double-stranded DNA dependent on an unmethylated, palindromic, asymmetric sequence, and in this example, it cuts the plasmid at a single site in the ampicillin-resistance gene. After that, the DNA is annealed with another fragment of DNA and this new recombinant molecule is used to transform <em>E. coli</em> cells. Transformation is a key step in DNA cloning because it occurs after restriction enzyme digestion and ligation treatments and transfers newly made plasmids into bacteria. <u>So these bacterias will have a new fragment of DNA, which still has a tetracycline resistance gene but it no longer has the ampicillin resistance gene because it was disrupted by the restiction enzyme</u>. Thereby, the antibiotic resistance phenotype is the tetracycline resistace.
Assuming this is for an EMT-B course, you would call your medical director for direction and give oral glucose if they are alert enough to control their own airway.
If this is for a Paramedic course, you will start an IV and administer D50 at a slow push.
Answer:
Saturated are full of hydrogen atoms, they´re solid at rooms temp, and they are produced by animals.
Unsaturated are liquid, have double bonds and they are produced by plants.
Trans fats are artificial, they are chemically changed, liquids turned into solids
Answer:
C
Explanation:
Due to the erosion of soils, no trees to protect the ground from drying out.
Water pressure and temperature varies at different depths of the ocean.
