Definition: "Copying of information from DNA to RNA which exit nucleus is known as transcription"
Explanation: Transcription is a vital process for living organisms, because it is a staring point for protein synthesis. It occurs inside the nucleus and is carried out by RNA polymerase enzyme. In eukaryotes RNA pol is of three types: these are RNA Pol 1, RNA Pol 2, and RNA Pol 3. In prokaryotes it is of only one type.
Steps in transcription: Transcription consists of three main steps
1. Initiation: In this step RNA pol attach to DNA template at 5 prime end in the promoter region to start the formation of complementary strand of RNA. In prokaryotes there is a region called
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Pribnow box, with the </span>consensus sequence<span> TATAAT present about 10 base pair away from site that serves as a transcription initiation. In eukaryotes the promoter region is more complex it contain activator and enhancer.
2. Strand elongation: </span><span>Once transcription is initiated, the DNA double helix unwinds and RNA polymerase reads the template strand, adding nucleotides to the 3′ end of the growing chain.
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3. Transcription Termination:
Terminator sequences are found close to the ends of non-coding sequences. Before termination polyadenylation occurs. <span>
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Answer:
Ashing process is done to evaluate the ash content present in petroleum or other proucts, so that they can be used for certain applications
The two major types of ashing process that includes dry ashing and wet ashing and can be performed using microwave systems or conventional means.
a. errors made in the preceding method while determining the ash content of buttermilk by conventional dry ashing are as following:
- Buttermilk should not immediatly put into muffle immediatley, it should be boiled first to avoid splatter.
- Temperature should be between 500 to 600 degree celcius at high temperature teh ash content can be loss.
- The sample should be ashed for 12-18 hrs
, more than this can damage the required ash content.
- platinum tip should be used in place of stainless steel because platinum has a lower concomitant tip impedance than stainless stell, so less reactive to ash.
- The ash should put in dessicator and not allow to kept in open because ash can react with the humidity in teh atmosphere.
b. The result that occur due to above errors such as the temperature, time, tongs and leaving ash in open will manipulate the final ash content and its application can be harmful for humans and for other purpose if it will be used.
Mitoses and Asexual Reproduction by cell division,one cell divides to become two
Answer:
The bond between the _second_________ phosphate group and the third__phosphate group is a high energy bond.
Explanation:
Adenosine triphosphate is a nucleotide with three phosphate groups. The second and third phosphate groups are bonded by high energy covalent bonds to the rest of the ATP molecule, which consists of a sugar, which is ribose, the first phosphate group, and the nitrogenous base which is adenine. All of the phosphate groups are negatively charged and repel each other, so a high energy covalent bond is necessary to attach these phosphate groups together and keep the forces from pulling them apart.