Question

You created three different derived strains from E.coli K12. One strain includes a deletion in a gene critical for biosynthesis of diaminopimelic acid, one strain lacks dnaQ, and the last strain lacks both. Describe experiments to clearly differentiate between these three strains through phenotypic assays alone (not just by sequencing the genomes or any derivative of genotyping). Include all necessary controls to interpert the experiment.

Answer 1

Answer:

For the strain that lacks DNAQ there will be slowed growth and there will more DNA breakage by Tunel assay

For the strain that lacks both The cells there will have a huge DNA breakage and less peptidoglycan.

Explanation:

One of the experiments that can be used to differentiate diaminopimelic acid is by observing the cell walls in which they are present because  are needed in the formation of cell walls. while to differentiate the three strains

For the strain that lacks DNAQ there will be slowed growth and there will more DNA breakage by Tunel assay

For the strain that lacks both The cells there will have a huge DNA breakage and less peptidoglycan.