You are inserting your gene of interest into the Lac Z gene in a plasmid also containing a tetracycline resistant gene. You plat
e your transformed bacteria on media containing tetracyline and X-gal (the LacZ substrate that can form a blue dye). What results would indicate a clone with recombinant plasmids?
Beta-galactosidase (also known as beta-gal or β-gal), will not be produced if a gene was cloned into the Lac Z gene. It is because β-galactosidase is produced by the activation of the lacZ gene, and thereby β-gal can not be produced in the absence of this lac operon. In consequence, X-gal will not be hydrolyzed by the β-gal enzyme in a reaction that releases a blue pigment, and thus the colony will be white. In gene cloning, X-gal enables to determine whether a cell is expressing a functional β-gal enzyme, which is known as the "blue/white screening method".
In economics, "natural resources" not created by people are called land.
The tools we use to create goods are called capital.
<u>EXPLANATION:</u>
In economics, natural resources not created by people in the factors of production are "land, labor, and the capital".
Out of these factors of production, land and labor are two things that not created by people. These two resources are utilized in the production process to create output, i.e "finished goods and services".
Land refers to the geographic area or territory which is all the gift of nature and is not created by human effort.
Labor refers to the individuals who utilize their skills, abilities, and efforts to manufacture or utilize the capital in the production of those goods and services.
Capital refers to the equipment, technology, factories, and tools which are used in the production and manufacturing of "goods and services". Hence, these are the factor of production.
