Answer:
The correct answer is -
II: lyse cells of interest, precipitate DNA
V. Digest (cut) genomic DNA with restriction enzyme
I. Digest (cut) phage (chromosome) with restriction enzyme so can ligate in the gene in next step!!
IV. Perform ligation (glue plasmid in!) reaction with genome fragments and phage.
III Introduce constructs via transformation into competent E. coli cells.
Explanation:
The total collection of the genomic DNA of an organism is known as the genomic library of the particular organism. In this library, the genomic DNA is inserted and stored in identical vectors.
The genomic library construction involved some major steps that are:
- Extract and purify DNA - lysing and separating the desired DNA of interest.
- Digest the DNA with a restriction enzyme - breaking or digesting DNA into fragments with restriction enzymes
- cut phage (chromosome) with restriction enzyme
- Insert the fragments of DNA into vectors
- introduce these recombinant a host bacterium by transformation, creating a DNA library.
<h3><u>Answer and explanation;</u></h3>
- This can be done by<u> DNA sequencing which is the process of determining the accurate order of nucleotides within a DNA molecule</u>.
- By doing a DNA sequencing for the two sequences of DNA from the two species it will help compare the number of random mutations which have occurred in a molecular clock in the two species - that is, seeing how different the sequences are.
- This will make it possible to infer how long ago the species diverged.
Species A: GTTGAGCTAGTATGGACC
Species B: GATAAGCTAGTAAGGTCA
These species differ in 5 nucleotides.
This number should be multiplied by the rate of mutations; that is;
5 × 15 million years =75 million years.
I believe the answer is C
Genetic mutations increase variety by adding uniqueness to the world. Genetic mutations can come in all shapes and sizes based on the chromosomes in cells. Perhaps someone was born with one blue eye and one green eye, or perhaps they have hair a certain, unusual color. Genetic mutations add variety to the world by making sure everyone isn't the same!!
