The answer is A; all of the above
After having one concussion you are more likely to have another — some doctorsestimate you increase your risk up to three times. There are two reasons why your risk increases: carelessness and brain chemistry.
When placed into an hypertonic solution, animal cells will shrivel up, while plant cells will stay firm thanks to their air-filled vacuole. In a hypotonic solution, the cells will take on water and appear more plump. In an isotonic solution, they'll stay the same.
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Answer:
By allowing the establishment of geological timescales, it provides a significant source of information about the ages of fossils and the deduced rates of evolutionary change. Radiometric dating is also used to date archaeological materials, including ancient artifacts.
Explanation:
hope it helps
