Running away is considered a crime for people who are under 18.
Answer:
The DNA must be able to separate its two strands in order to be connected to the sliding clamp.
For this to occur, the action of the protein components: helicase and topoisomerase is necessary.
Explanation:
The sliding clamp is a protein that participates in DNA replication. This protein is responsible for forming a "ring" around the single strand of DNA, allowing the polymer DNA (DNA replicating enzyme) to be firmly suited to the strand and to be able to create the new DNA strands. The sliding clamp is also responsible for the DNA polymerase decoupling from the DNA at the end of replication.
For the sliding clamp to work correctly, it is necessary for the DNA to unravel and release its two strands. This process needs the help of the Helicase enzymes, which are responsible for breaking the hydrogen bonds that hold the two DNA strands together, and, for topoisomerase, which is the enzyme that prevents single strands of DNA from creating torsions in their extension.
Answer:
Self-made entrepreneur
Explanation:
A self-made entrepreneur is somebody who started his own business from scratch, without any foreign help. The business usually starts as an idea which is often something the entrepreneur is passionate about. In case of Jules Sylvester the passion were his reptiles. He cleverly turned his passion into his business, knowing that his knowledge and his love for animals are invaluable for his enterprise. As many self-made entrepreneurs, he started his venture without any financial help from others, and he might have faced various risks and obstacles at the beginning.
Answer: Nurseries, preschools and individual childcare providers such as nannies
Explanation: The cost of a nursery varies depending on the location, the length of childcare time needed and the type of services provided.
