Answer:
B. Proteins complexed with DNA to form nucleosomes- Histones
I. Used in chain terminator procedure to stop chain elongation- ddNTPs
H. Separation of DNA fragments based on polyanionic nature- Gel electrophoresis
F. Substrates for translation- mRNA
J. A DNA gel for sequencing shows the base sequence of GATCGGA reading from bottom to top. The template sequence can be determined using this information 5' - TCCGATC
Explanation:
1. The DNA in the chromatin is packaged and ordered into structural units known as nucleosomes by theirformation of complexes with proteins called Histones. Histones are small basic proteins rich in the amino acids arginine and lysine.
2. In the chain termination procedure of DNA sequencing, chain elongation is terminated at several points whenever the dideoxynucleotides, ddNTPs are added to the growing sequence of the newly synthesized DNA strand. Because the ddNTPs lack an OH group and 3' position, new nucleotides cannot be added.
3. Gel electrophoresis is a separation technique used to separate DNA fragments based on ability to migrate an electric field. The DNA molecules are negatively-charged (polyanionic) and are separated based on their size with smaller molecules migrating fastest than bigger ones.
4. The messenger RNA, is synthesized from the part of the DNA coding for a functional protein. They serve as the template from which the ribosomes read to make proteins. Thus, they serve as the substrates for translation.
5. The reading of the base sequence from an autoradiogram (DNA gel for sequencing) is from top to bottom in the 5' to 3' direction. Since the template is complementary to it, it will read in the 3' to 5' direction CTAGCCT or TCCGATC in the 5' to 3' direction.
