Gram's staining is a differential staining technique that employs a primary stain like crystal violet and a counter stain like safranin along with the decolourizing agent alcohol and a mordant called the Gram's iodine.
Iodine is a mordant added after the primary stain. It fixes the stain by combining with it to enchance the staining ability. This forms an insoluble crystal violet iodine complex appearing purple under the microscope. These microorganisms are classified as Gram positive.
If addition of iodine is skipped, crystal violet is not fixed on the slide and the insoluble complex is not formed. The cells are decolourized by alcohol and are stained by the counter stain safranin making the Gram positive cells wrongly indentified as Gram negative due to its pink colouration. Thus, the slide will show all the cells as pink coloured Gram negative cells.
The weak bonds are called hydrogen bonds and they are used to connect the hydrogen atom to oxygen atom in order to share electrons.
Answer:
2
Explanation:
Fats are the slowest source of energy but the most energy-efficient form of food. Each gram of fat supplies the body with about 9 calories, more than twice that supplied by proteins or carbohydrates.
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Nucleus, Mitochondria, vacuole
