Answer and Explanation:
The steps for construction of the expression plasma
- STEP 1 : Introducing Notl and Hindlll confinement sites at the ends of the gene of interest by means of PCR
- STEP 2 :Digest the plasmid and DNA encoding gene with the Notl and Hindol confinement endonucleuses.
- STEP 3 : Ligate the cut plasmid and DNA fragment
- STEP 4 : Change the plasmid into E.coli for expression
- STEP 5 : Clean the protein by utilizing a column of immobilized

Explanation:
im pretty sure its They ward off disease-causing organisms by competing for space and nutrients on and inside the body. ... Scientists and doctors can even utilize prokaryotes to help the human body. The two key components of ecosystem stability are resilience and resistanc
It is called <span>stroke for your question</span>
Answer:
Parallel to the wave motion
Explanation:
Longitudinal waves are waves that propagates in the direction of the wave motion.
Transverse waves are perpendicular.
During photosynthesis, the energy used to pump protons comes from ______light_____, whereas in cellular respiration it comes from ______NADH/FADH₂_______.
<h3>What are the steps in photosynthesis?</h3>
- The first step in photosynthesis is the absorption of light by chlorophyll bound to chloroplast thylakoid proteins. The absorbed light energy is used to remove electrons from electron donors such as water to form oxygen.
- The electrons are then transferred to the primary electron acceptor, quinine (Q.). Electrons are further transferred from the primary electron acceptor to the final electron acceptor (usually NADP⁺).
- Proton transfer from the thylakoid lumen to the stroma via the F₀F₁ complex generates ATP from ADP and Pi.
- The NADP and ATP produced in steps 2 and 3 provide the energy, and the electrons power the process of reducing the carbon to a six-carbon sugar molecule.
The first three steps of photosynthesis, are directly dependent on light energy and are thus, called light reactions, while the reactions in the last step are independent of light and thus are termed dark reactions.
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