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Mekhanik [1.2K]
3 years ago
9

¿Cuál es el fundamento de la prueba de sensibilidad por difusión?

Biology
1 answer:
torisob [31]3 years ago
5 0

Answer:

La identificación del agente patógeno mediante el antibiótico/quimioterápico correspondiente

Explanation:

La prueba de sensibilidad por difusión en agar (también conocida como método de Bauer & Kirby) es ampliamente utilizada en laboratorios clínicos. La técnica consiste en realizar un antibiograma el cual permite determinar la susceptibilidad del microbio frente a drogas específicas. El antibiograma consiste en depositar sobre placas de petri conteniendo microbios cultivados en agar diferentes discos de papel impregnados cada uno con un antibiótico específico. De este modo, la prueba de sensibilidad por difusión en agar permite determinar la sensibilidad del patógeno con respecto a un antibiótico específico y/o droga quimioterápica.

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REAL ANSWERS PLEASE !!!<br> Describe the steps of transcription
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Answer

The various steps in the transcription and translation process of protein synthesis are described below.

Explanation:

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Transcription: It is the first process in protein synthesis which occurs in the cell nucleus where a single-stranded messenger RNA (mRNA) is created using a DNA strand and the genetic instructions in DNA are transferred to this mRNA. The steps in transcription are initiation, elongation, and termination. The beginning process known as initiation occurs when an enzyme RNA polymerase binds to a promoter (region of a gene) and the DNA unwinds. One of the DNA strands acts as a template and the enzyme reads the bases in the template DNA strand.

The next step is elongation, where the RNA polymerase builds a strand of mRNA by the addition of nucleotides using complementary base pairs. Here, adenine (A) in the DNA binds to uracil (U) in the RNA. Termination is the last step in which the transcription process ends when the RNA polymerase comes across a termination sequence in the gene. Thus, the completed single-stranded mRNA detaches from DNA.

Translation: It is the second process in protein synthesis which occurs in the ribosome of the cell where the genetic information in mRNA is used to create a protein from amino acids. A triplet of nucleotides is called a codon and they define amino acids. There are 64 possible codons and the codon, AUG acts as the start codon which initiates translation in addition to specifying the amino acid methionine. In the initiation step, the first amino acid in the polypeptide chain is brought by transfer RNAs (tRNAs) to bind to the start codon of mRNA. During elongation, each type of tRNAs in the cytoplasm bound to a specific codon on the mRNA template and adds the corresponding amino acid to the polypeptide chain. Stop codons (UAA, UAG, or UGA) terminate protein synthesis and release the polypeptide.

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