Answer: Tetracycline.
Explanation:
A plasmid is a small circular DNA molecule found in bacteria that is separate from the bacterial chromosome and replicates independently of it. They encode for certain genes that play a key role in antibiotic resistance. <u>Restriction enzymes are endonucleases that catalyze the cleavage of phosphodiester bonds in different regions located within a DNA strand</u>. PstI is an example of an endonuclease, it is a type II restriction enzyme produced by the microorganism <em>Providencia stuartii</em> that possesses a restriction target in double-stranded DNA dependent on an unmethylated, palindromic, asymmetric sequence, and in this example, it cuts the plasmid at a single site in the ampicillin-resistance gene. After that, the DNA is annealed with another fragment of DNA and this new recombinant molecule is used to transform <em>E. coli</em> cells. Transformation is a key step in DNA cloning because it occurs after restriction enzyme digestion and ligation treatments and transfers newly made plasmids into bacteria. <u>So these bacterias will have a new fragment of DNA, which still has a tetracycline resistance gene but it no longer has the ampicillin resistance gene because it was disrupted by the restiction enzyme</u>. Thereby, the antibiotic resistance phenotype is the tetracycline resistace.
Answer:
Pandemics occur for two reasons. The first is a change in the infectious agent and the second is in human patterns. Both involve exposing people to agents they don’t have resistance to. Diseases like SARS, avian flu, and the famous Spanish flu all involved a mutation in the virus that resulted in a new disease that no one had resistance too. Examples of the second are when new groups of humans make contact, like small pox amongst native Americans, and possibly the black death.
Explanation:
That is all up to ones opinion no one really knows because no one that is living now was alive then all we can go by is secondary sources to let us know
