Answer:
Can I see the options if it have any
Explanation:
Answer:
The smallest size tube that will hold the entire reaction is 0.5ml
Explanation:
25ul (DNA sample) + 25ul (enzyme) + 50ul (buffer) + 400ul (water) = 500ul total
since 1000ul=1ml, then 500ul = 0.5ml
Answer:
The device that mostlikely would not be used P20
Explanation:
P20 is a pipette used to measure and sample volumes of 20ul maximum.
Answer:
The reaction mix will have 400 units of the enzyme.
The working dilution will be 0.8 Units/ul.
Explanation:
The mix has 20ul of the enzyme which is 20Units/ul. Then we have 20ulx20Units/ul= 400 Units of enzyme in the mix.
Since the final volume is 500ul, the final concentration 400Units/500ul=0.8 Units/ul.
Answer:
Used the C1V1 = C2V2 method for calculations
Explanation:
Component Calculation Volume of Stock to be added
FBS: 100*V1 = 10*25 2.5 mL
Glutamine: 200*V1 = 2*25 0.25 mL
Penicillin: 10000V1 = 100*25 0.25 mL
Streptomycin: 10000V1 = 100*25 0.25 mL
NB: C1 = Stock Concentration; V1 = Volume of stock required; C2 = Final concentration required; V2 = Final volume required
Answer:
Yes, it is
Explanation:
It is very much analogous. Just as in the analogy explained, DNA isn't visible to the naked eye when it is alone or single, or just one. On the other hand, it is very much easier to see when the DNA are in a cluster, just like in the cotton example.
Succinctly put, the example is analogous to DNA extraction
Phase 1 of the creation of life on earth was that life on earth was from nothing. Small molecules were formed from atoms of carbon and hydrogen. Earth does not have any oxygen rather it contains CO2, methane, nitrogen, hydrogen and ammonia where they were from the eruptions of volcanoes.
