1. Samples of a microbial colony obtained from the natural environment are not pure culture. They are mixed cultures because the environment consists of an array of microorganisms and not one specific organism. A sample obtained from the natural environment such as water or soil may contain several species of microbes that need to be further isolated to obtain a pure culture.
2. Isolation streak plate technique involves the dilution of the sample several folds and then streaking a small amount using a sterile inoculation loop on an agar plate in a zig-zag manner. This is done to obtain a pure culture of bacteria from a mixed population by separating the microprocessor. When the sample is diluted into a small concentration and is streaked onto agar placed, widely spaced, discrete colonies of bacteria can be observed. These isolated colonies can be further cultured to obtain pure cultures by picking and re-streaking them on fresh agar plates.
3. A microbial colony is considered a pure culture because it consists of single species. A single species of bacteria cluster and form an isolated colony on an agar plate which can be further plated on fresh medium.
3-1 - If your agar plate shows discrete uncluttered colonies then you have obtained an isolated colony. The following picture might help you to understand.
3-2. If you subculture isolated microbes into a sterile nutrient broth without any contamination, it will still be considered pure culture because it is single isolated species in a sterile aseptic environment of the nutrient broth.
3.3. A pure culture can become a mixed culture due to contamination. This could be caused by airborne bacteria if your conditions are not aseptic. Contamination is possible if the work area is not clean and sterile or if the glassware is not sterile. The contamination can transform your pure culture into a mixed culture and is hence problematic. So, it is very important to maintain a sterile and aseptic environment, glassware, and instruments.
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Answer:
Density = 388.57 kg/m³
Explanation:
Given the following data;
Mass = 52457 grams to kilograms = 52457/1000 = 52.457 kilograms
Volume = 13.5 cm to meters = 13.5/100 = 0.135 m
To find the density;
Density can be defined as mass all over the volume of an object.
Simply stated, density is mass per unit volume of an object.
Mathematically, density is given by the formula;
Substituting into the formula, we have;
Density = 388.57 kg/m³
Answer:
According to the information on the map, the natural resource that is found in both Mexico and Canada are:
Copper Iron and silver.
Explanation:
The reasons behind this answer are the following. In the first place, Silver is located in Ontario, Quebec, and New Brunswick in Canada, while in Mexico it is located In Jalisco, Aguas Calientes, and San Luis. Copper is found in basically all its territory but principally Ontario and Quebec. While it is found in San Luis and Oaxaca. Finally, iron is found in Quebec, New Foundland and Labrador in Canada, and San Luis, Oaxaca, and Michoacan. The three elements are found in both countries.
Answer: The correct answer is : a. John augustus
Explanation: Augustus was "the Father of probation" who in 1841 attended the police to rescue a common drunk who when he appeared in court was a sober man who had changed his appearance and behavior and was accompanied by Augustus. John Augustus thus began an 18-year career as a probation officer.
i think its southwest... i might be wrong tho but i think its right
