Question

PCR amplification utilizes ________ template to produce a ________ product. For this question, select the answer that best describes the process as a whole: the nature of the template you used and the nature of the final product produced in your PCR analysis that you ran out on a gel will apply here. (Ref: slide 5

Answer 1

Answer:

Single stranded template to produce a double stranded product.

Explanation:

Polymerase chain reaction (PCR) is a technique in the laboratory that is used to make many copies (millions or billions) of a particular region of deoxyribonucleic acid (DNA).

The reaction utilizes primers which are short pieces of single stranded oligonucleotide templates to yield a double stranded DNA.

Two primers are used in each PCR reaction, and they are designed to identify the target region to amplified they bind to opposite strands of the template DNA at the edges of the region to be copied. The template DNA is copied and a complementary DNA is produced. The end product of the reactions a double stranded DNA that is produced from the template dna. The new DNA is the exact copy of the template dna.