Answer:
The correct answer is b.Amplify the gene using PCR. Insert the gene into a plasmid vector. Transform the vector into the bacteria.
Explanation:
If I have a very small amount of gene for a fluorescent protein than the first step is to amplify the gene so that appropriate protein can be produced. PCR is the instrument that is used to amplify the protein.
So after amplification of the gene, the plasmid vector will be used in which the gene will be inserted because the plasmid vector is used to insert this gene in host cells where protein will be expressed.
The final step will be to transform bacteria with recombinant plasmid so that plasmid can make its copy and express a fluorescent protein in bulk.
Cytoplasm. This photo should help explain it!
The question is incomplete as it does not have the options which are:
A) single-stranded complementary tails
B) blunt ends
C) poly-A sequences
D) 5' cap
E) interference
Answer:
A) single stranded complementary tails
Explanation:
Restriction endonuclease is the enzyme which cuts the DNA sequence in the internal sequence.
The endonuclease enzyme can cut the DNA sequence in a way that it can form the cuts with the single-stranded overhangs called sticky ends and without overhangs called blunt ends.
The sticky ends are produced when the enzyme makes cut at the single strand and then makes the cut at between the same base at the nitrogenous base. This type of asymmetrical cut forms the single-stranded overhangs which can form the complementary base pairs easily.
Thus, Option-A is correct.
Answer:The answer would be C.
Explanation:
Law of superposition, a major principle of stratigraphy stating that within a sequence of layers of sedimentary rock, the oldest layer is at the base and that the layers are progressively younger with ascending order in the sequence.
Answer:
Explanation:
They are found only in the zone of maturation, and not the zone of elongation, possibly because any root hairs that arise are sheared off as the root elongates and moves through the soil. Root hairs grow quickly, at least 1μm/min, making them particularly useful for research on cell expansion.