Macbeth drugged the guards with wine, which led to her stabbing Duncan. Afterwards she decides to frame the guards.
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The purpose of a literary analysis essay should be to evaluate and analyze how the author portrays his message to the audience. In addition to answering "what" the author's message is, you also need to answer "how" the author does it. Some examples include imagery, similes, metaphors, the word choice, etc.
The theme is about letting go at one point and following your heart.