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ahrayia [7]
3 years ago
5

You have just trypsinized your monolayer cells growing in a T25 flask. Once detached you resuspended all of your cells in media.

If you were to subculture some of your cells into a new T25 cells by taking 1.5 mL of cell suspension from a total of 3 mL and transferring it into a new T25 flask containing 8 mL of fresh media, your final dilution would be:
Biology
1 answer:
Serga [27]3 years ago
8 0

Answer:

Final dilution is   \frac{1}{6.33}

Explanation:

Given

Volume of suspension = 3 mL

Volume of subculture = 1.5 mL

Volume of fresh media = 8 mL

Final dilution

\frac{1.5}{8+1.5}

Final dilution

 = \frac{1.5}{9.5}\\\\= \frac{15}{95} \\= \frac{1}{6.33}

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