Question

You have just trypsinized your monolayer cells growing in a T25 flask. Once detached you resuspended all of your cells in media. If you were to subculture some of your cells into a new T25 cells by taking 1.5 mL of cell suspension from a total of 3 mL and transferring it into a new T25 flask containing 8 mL of fresh media, your final dilution would be:

Answer 1

Answer:

Final dilution is   $\frac{1}{6.33}$

Explanation:

Given

Volume of suspension $= 3$ mL

Volume of subculture $= 1.5$ mL

Volume of fresh media $= 8$ mL

Final dilution

$\frac{1.5}{8+1.5}$

Final dilution

 $= \frac{1.5}{9.5}\\\\= \frac{15}{95} \\= \frac{1}{6.33}$