First question is D poor farming practices
Direct effects, as the name suggests, while not manipulated or communicated by a third party, deal with the direct influence of one entity on another. Indirect effects can be described as the influence, mediated or passed on by a third, of one organism or species on another.
Answer:
The introns of protein-encoding nuclear genes of higher eukaryotes almost invariably begin (5') with GT and end (3') with AG. In addition, the 3'subterminal A in the “TACTAAC box” is completely conserved; this A is involved in bond formation during intron excision.