You work in a lab that is studying PIWI proteins. You isolate piRISCs from cells. Based on what you know about how piRISCs silen
ce transposable elements, what enzymatic activities do you anticipate will be associated with the proteins you've isolated (either in the piRISCs themselves, or in proteins that may have been associated with the piRISCs)? a. Nuclease activityNuclease activity
b. DNA methylaseDNA methylase
c. ProteaseProtease
d. DNA ligaseDNA ligase
e. Kinase
The correct answer is - a. Nuclease activity and b. DNA methylaseDNA methylase.
Explanation:
The binding of the miRNA with the complementary DNA or mRNA sequence by either methylation of DNA, or by nuclease activity of the proteins, by the help of RISC complex as it brought them together In piRISCs complex. This complex goes and binds to the nucleotides to cause the silencing of the mRNA or the DNA sequence.
Thus, the correct answer is - a. Nuclease activity and b. DNA methylaseDNA methylase.
Derived from the inner cells of a human blastocysts , a very early human embryo. At the blastocyst stage, five to 10 days after fertilization, the embryo is a cluster of 100-200 cells
