Answer:
42,5 mL
Explanation:
We need to use the serial dilution formula beacuse we start with a stock concentrate solution and we need to prepare a new less concentrated one.
![DF=\frac{Vi}{Vf}](https://tex.z-dn.net/?f=DF%3D%5Cfrac%7BVi%7D%7BVf%7D)
<u>DF in the dilution factor, Vi is the initial volume and Vf is the final volume.</u>
The first step is to have the same measurment unit so we need to convert 345 µg to mg.
we know that 1 µg equals 0,001 g, hence:
![345 µg = 0,345 mg](https://tex.z-dn.net/?f=345%20%C2%B5g%20%3D%200%2C345%20mg)
now the final volume is 0,345 mg protein/ mL and the inital volume is 15mg protein/mL, both of them are in the same unit so we can use the formula
![DF= \frac{15mg protein/mL}{0.345mg protein/mL}](https://tex.z-dn.net/?f=DF%3D%20%5Cfrac%7B15mg%20protein%2FmL%7D%7B0.345mg%20protein%2FmL%7D)
![DF= 43,5 mg protein/ mL](https://tex.z-dn.net/?f=DF%3D%2043%2C5%20mg%20protein%2F%20mL)
Now since the question said that we already have 1.0mL of the amylase stock solution we need to subtract that 1.0mL to the 43,5 mg protein/mL
![43,5mL-1,0mL = 42,5 mL](https://tex.z-dn.net/?f=43%2C5mL-1%2C0mL%20%3D%2042%2C5%20mL)
So, we need 42,5 mL of diluting buffer if we want a final concentration of 345 µg protein/mL (0.345 mg protein/mL)
Answer;
They have revealed new information about cell structure and processes.
Explanation;
-The Cell Theory states that, All living things are made up of cells, Cells are the basic units of structure and function in living things, and that new cells are produced from existing cells.
-Most microscopes use lenses to magnify the image of an object by focusing light or electrons.
-The Invention of the Microscope helped the development of the cell theory because it allowed the scientists to actually discover that everything was made up of cells, and what cells do to come up with that theory. If the scientists did not see that cells existed, they could not have observed the cells easily and they would not be able to construct the cell theory.
Answer: Anesthesia
Explanation:
Anesthesia was used in 95% of Civil War surgeries. Sulfuric ether had been discovered in Europe in the 1830's and was first used as an anesthetic by William Morton in 1846. After a successful public demonstration it became widely used in surgery.